Biotrofix Acute Model Rat - Stroke Animal Models
ACUTE STROKE NEUROPROTECTION MODEL
MODEL: PERMANENT SUTURE OCCLUSION (FILAMENT OCCLUSION)
OF MIDDLE CEREBRAL ARTERY (MCA)
TEST NUMBER: BTFX-A01
CATEGORY: Permanent focal ischemia model
SPECIES: Mature Wistar or Sprague-Dawley rats
APPLICATION: Testing neuroprotective agents in a model of acute focal stroke (permanent
occlusion)
METHOD:
Mature rats are allowed free access to food and water before surgery.
Animals are anesthetized with halothane or isoflourane by inhalation or
chloral hydrate by i.p. injection. Rectal temperature is maintained at 37.5
± 0.5° C using a heating blanket connected to a temperature controller.
Blood pressure and blood gases can be monitored though the femoral
artery.
Under the operating microscope, the bifurcation of the right common
carotid artery is exposed through a midline incision in the neck. A 4-0
monofilamnent nylon suture with its tip rounded near a flame is
introduced into the right external carotid artery and advanced into the
internal carotid artery for a length of 17~20 mm from the bifurcation.
These methods place the tip of the suture at the origin of the anterior
cerebral artery, thereby occluding the middle cerebral artery. The suture is
left in place until death.
Following MCA occlusion, animals are allowed to awaken from
anesthesia. Surgical mortality is <10% in this model.
At 24 h after ischemia, animals are assessed by a brief rating scale for
neurological dysfunction. Animals are then killed by an overdose of
chloral hydrate, and brains are removed for infarct volume assessment.
ENDPOINTS: Infarct volume; short-term behavioral studies
DESCRIPTION OF INFARCTION:
This method produces a large infarction in the distribution of the MCA,
involving a large portion of the lateral cerebral cortex and underlying
white matter and striatum. The cortex represents the ischemic penumbra
in this model.. Animals typically survive for only a day or two, making
this model appropriate for short-term neuroprotection studies.
REFERENCES:
1. Fisher, M., M.-E. Meadows, T. Do, J. Weise, V. Trubetskoy, M.
Charette and S. P. Finklestein (1995). “Delayed treatment with
intravenous basic fibroblast growth factor reduces infarct size following
permanent focal cerebral ischemia in rats.” J. Cereb. Blood Flow Metab.
15: 953-959.
2. Ren, J. and S. P. Finklestein (1997). “Time dependence of infarct
reduction by intravenous basic fibroblast growth factor (bFGF) following
focal cerebral ischemia in rats.” Eur. J. Pharmacol. 327: 11-16. |
